Alix Molecular Weight Products Primary Antibodies R Recombinant Alix E6P9B Rabbit mAb 92880 Citations 51 Filter WB IP IF Confocal immunofluorescent analysis of HCT 116 cells left positive and HCT 116 Alix knockout cells right negative using Alix E6P9B Rabbit mAb green Blue pseudocolor DRAQ5 4084 fluorescent DNA dye
Product name Anti ALIX antibody See all ALIX primary antibodies Description Mouse polyclonal to ALIX Host species Mouse Tested applications Suitable for WB ICC IF more details Species reactivity Reacts with Human Immunogen Recombinant full length protein within Human ALIX Description Rabbit polyclonal to ALIX Host species Rabbit Tested applications Suitable for WB IHC P more details Species reactivity Reacts with Human Predicted to work with Mouse Rat Chicken Orangutan Immunogen Synthetic peptide corresponding to Human ALIX aa 450 550 conjugated to keyhole limpet haemocyanin Peptide available as ab88953
Alix Molecular Weight
Alix Molecular Weight
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Alix
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Alix
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Molecular Weight of Alix 95 kDa Positive Controls HeLa whole cell lysate sc 2200 K 562 whole cell lysate sc 2203 or HeLa nuclear extract sc 2120 STORAGE Store at 4 C DO NOT FREEZE Stable shipment Non hazardous No MSDS required DATA 215 K 114 K 88 K A B C D E F Alix Alix 3A9 Alexa Fluor 647 sc 53538 AF647 Overview Product name Anti ALIX antibody See all ALIX primary antibodies Description Rabbit polyclonal to ALIX Host species Rabbit Specificity Replenishment batches of our polyclonal antibody ab88388 are tested in WB Previous batches were additionally validated in ICC
Alix is involved in the concentration and sorting of cargo proteins of the multivesicular body for incorpoation into vesicles CHROMOSOMAL LOCATION Genetic locus PDCD6IP human mapping to 3p22 3 Pdcd6ip mouse mapping to 9 F3 SOURCE Alix 1A12 is a mouse monoclonal antibody raised agianst full length Alix of human origin PRODUCT Molecular Weight of Alix 95 kDa Positive Controls K 562 whole cell lysate sc 2203 Jurkat whole cell lysate sc 2204 or WEHI 231 whole cell lysate sc 2213 STORAGE Store at 4 C DO NOT FREEZE Stable for one year from the date of shipment Non hazardous No MSDS required RESEARCH USE For research use only not for use in diagnostic DATA
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Product Description Rabbit anti Human ALIX antibody recognizes ALIX also known as PDCD6IP AIP1 HP95 DRIP4 or ALG 2 interacting protein X This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis in intralumenal endosomal vesicle formation and in enveloped virus budding Disclaimer note The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications post translation cleavages relative charges and other experimental factors Alix encodes a protein thought to participate in programmed cell death Studies using mouse cells have shown
Only in absence of the reducing agent we could detect bands of 200 kDa in addition to the Alix monomer of 100 kDa in the membrane organelle and more evidently in the cytoskeleton subcellular fractions These high molecular weight forms were not visible in the Alix samples Fig 1 A and S1 A A ALIX Bro1 V and B ALIX V analyzed by MALLS indicates molecular weights of 80 and 160 kDa for ALIX Bro1 V and 40 and 80 kDa for ALIX V left y axis refractive index the molecular weight is blotted on the right axis in a logarithmic scale x axis shows the elution volume of the peaks
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Alix Molecular Weight - Abstract Nature of exosome secreting cells determines exosome content and function ALIX involved in exosome biogenesis promotes cell degeneration Here ALIX was knocked out iPSC ALIX and overexpressed iPSC ALIX3 in induced pluripotent stem cells iPSCs using CRISPR Cas9 and lentiviral transduction respectively and the secreted exosomes were analyzed Exosomes from iPSC ALIX